3D Bioprinting of a Bioactive Composite Scaffold for Cell Delivery in Periodontal Tissue Regeneration
نویسندگان
چکیده
Hydrogels have been widely applied to the fabrication of tissue engineering scaffolds via three-dimensional (3D) bioprinting because their extracellular matrix-like properties, capacity for living cell encapsulation, and shapeable customization depending on defect shape. However, current hydrogel show limited regeneration activity, especially in application periodontal regeneration. In this study, we attempted develop a novel multi-component that possesses good biological can wrap cells 3D regenerate soft hard tissue. The consisted gelatin methacryloyl (GelMA), sodium alginate (SA) bioactive glass microsphere (BGM), which was first processed into by cell-free printing evaluate its printability vitro performances. 3D-printed showed uniform porous structures swelling capability. BGM-loaded scaffold exhibited biocompatibility, enhanced osteogenic differentiation, apatite formation abilities desired mechanical strength. composite further as bio-ink load with mouse bone marrow mesenchymal stem (mBMSCs) growth factors (BMP2 PDGF) wrapped still maintained cellular vitality after differentiation repair capabilities BMP2- PDGF-loaded scaffolds. It noted transplantation cell- factor-laden Beagle dog defects, significant gingival tissue, ligament, alveolar detected. Importantly, reconstructed structure established treatment group eight weeks post-transplantation containing factors. conclusion, developed applicable reconstruction defects.
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ژورنال
عنوان ژورنال: Biomolecules
سال: 2023
ISSN: ['2218-273X']
DOI: https://doi.org/10.3390/biom13071062